Welcome to the IDT family!
Your product is now available from Integrated DNA Technologies.
Many of the Swift products you have grown to love are now part of our new complete portfolio, xGen™ NGS. Through this new partnership we are pleased to offer you comprehensive next generation sequencing solutions.
xGen NGS—made for you.
Unsure of what products are available? Or, perhaps you’d like guidance on which products are compatible? If so, try our xGen NGS Solutions Builder Tool today.
Processing
An improved solution for generating large CRISPR HDR knock-ins
CRISPR-based homology-directed repair (HDR) is an invaluable tool to facilitate specific mutations in a genomic region of interest in research studies. While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically generated donor templates.
What you will learn:
- How dsDNA donor templates with novel end-modifications improve the frequency of HDR and reduce homology-independent (blunt) insertion events.
- How the addition of Alt-R HDR Enhancer V2, a small molecule compound, can further improve HDR rates.
- Design considerations when using large dsDNA templates for successful HDR experiments.
Published on: June 06, 2022