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An improved solution for generating large CRISPR HDR knock-ins

An improved solution for generating large CRISPR HDR knock-ins

CRISPR-based homology-directed repair (HDR) is an invaluable tool to facilitate specific mutations in a genomic region of interest in research studies. While many methods have been reported for improving HDR efficiency, achieving precise changes via HDR remains a challenge, particularly for large knock-ins. These insertions can be generated via HDR using enzymatically generated donor templates.

What you will learn:

  • How dsDNA donor templates with novel end-modifications improve the frequency of HDR and reduce homology-independent (blunt) insertion events.
  • How the addition of Alt-R HDR Enhancer V2, a small molecule compound, can further improve HDR rates.
  • Design considerations when using large dsDNA templates for successful HDR experiments.

Published on: June 06, 2022

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.