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Troubleshooting qPCR: What are my amplification curves telling me?
Quantitative PCR (qPCR) is the method of choice for accurate estimation of gene expression. Part of its appeal for researchers comes from having a protocol that is easy to execute. However, when your reactions don't yield ideal amplification, troubleshooting the "why" can be challenging. Factors including sample quality, template quantity, master mix differences, assay design, and incorrect primer or probe resuspension. When troubleshooting, analysis of your amplification curves can provide clues to improve your results. This webinar covers a variety of problematic qPCR issues and how they affect amplification curves.