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Modification Highlight: ZEN™ Internal Quencher

Use of the ZEN Quencher as a second, internal quencher in qPCR 5’-nuclease assay probes provides greater overall dye quenching, lowering background, and increasing signal detection. When incorporated into oligonucleotides, it also serves to strengthen duplex formation and block exonuclease digestion, while remaining nontoxic to cells. Thus the ZEN Quencher can be useful in steric blocking antisense oligonucleotide applications.

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Multiplex qPCR—How to Get Started

Learn how multiplex qPCR can save sample, reagent cost, and time. The article provides recommendations for multiplex qPCR assay design and experimental setup.

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qPCR Terminology—What Does It Mean?

Definitions of some of the most commonly used terms and distinctions encountered in qPCR experiments.

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A Conversation About qPCR with Jo Vandesompele

An interview with Dr Jo Vandesompele, internationally recognized expert on quantitative PCR, discussing the common issues researchers face when using qPCR, and the future direction of qPCR technology.

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Sample preparation for successful qPCR

Considerations for qPCR sample preparation for obtaining accurate and consistent results.

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Steps for a Successful qPCR Experiment

Considerations for 5′ nuclease assay design and experimental setup to help you obtain accurate and consistent results.

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Identifying Stem Cell Differentiation Biomarkers

Scientists at ReGenesys are using PrimeTime qPCR Assays in multiplex qPCR experiments to identify biomarkers that allow them to stage cells as they de-differentiate into stem cells. These researchers work with a bone marrow derived cell line, called MultiStem, a primitve mesenchymal stem cell variant. MultiStem cells have the potential to be used as an off-the-shelf product to treat several diseases.

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Using PrimeTime qPCR Assays in Multiplex Experiments

IDT PrimeTime qPCR Assays offer multiple dye/quencher combinations and primer/probe ratios to simplify the multiplex experiment design.

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PrimeTime Predesigned qPCR Assays Selection Tool

IDT’s design engine incorporates many parameters to produce the best possible PrimeTime Pre-designed qPCR Assay.

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Tips for Using BLAST to Locate PCR Primers

Need a quick way to find the location of primers within a gene or the expected size of the resultant PCR product? In this tip we show you how to get this information using BLAST.

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