In some cases, obtaining RNA or DNA for PCR cloning of a gene that is not well studied can be a significant challenge. In cases where the gene is critical to the study, sometimes the only option is to assemble the sequence using synthetically produced DNA.
In this paper, the Micalcl protein is one of several extracellular matrix proteins that are examined for their association with prion resistance. Cloned sequences for Micalcl were not readily available. As a fast and reliable alternative to finding or making template DNA, the authors assembled 5 gBlocks Gene Fragments to assemble the ~2 kb coding sequence for Micalcl, using the Gibson Assembly® method.