gBlocks® Gene Fragments—Related DECODED Articles

A high throughput, high resolution melting protocol for identifying single-nucleotide polymorphisms

Bruzzone CM, Tawadros PS, et al. (2013) Enhanced Primer Selection and Synthetic Amplicon Templates Optimize High-Resolution Melting Analysis of Sin­gle-Nucleotide Polymorphisms in a Large Population. Genet Test Mol Biomarkers, Epub ahead of print.

Single nucleotide polymorphisms (SNPs) are commonly studied for their contribution to genetic variation and disease. In this article, Bruzzone et al. present a novel method for evaluating SNPs in a fast, high-throughput protocol. The protocol relies on careful primer design, and a “cloning fragment length double-stranded DNA control template” to verify the identity of homozygous peaks in the melt-curve analysis for SNP detection. Sequence-verified gBlocks® Gene Fragments were an ideal solution for the melt-curve controls in this study, and were central to the SNP analysis.

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