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NGS Discovery Pools

Days vs. weeks might mean everything

NGS Discovery Pools are high-fidelity, individually-synthesized, 5′-biotinylated oligos for targeted NGS. Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. These nucleic acid probes can be used for creating custom capture panels that can be optimized, expanded, and combined with other panels. Improvements to our established manufacturing process allow you to build custom panels quickly at a fraction of the cost of conventional custom panels.

  • Fastest custom panel turnaround time in the industry
  • Affordable for any size panel
  • Easy design for even coverage across your targets

Ordering

NGS Discovery Pools

For information about pricing or to get a quote, please contact IDT sales.

For panel designs requiring expert analysis, please contact IDT support.

If you already have your custom panel design, upload your sequences.

NGS Discovery Pools are pooled, high-fidelity, single-stranded DNA probes for targeted next generation sequencing. Create custom capture panels that can be optimized, expanded, and combined with other panels. Panels ship in as few as 5 business days (<50,000 probes).

Proprietary DNA synthesis equipment permits rapid, high-quality synthesis of nucleic acids. This platform is the same proprietary synthesis platform used to make IDT Ultramers. It uses an "extra rich" synthesis cycle for long oligos. Along with this refined synthesis cycle, these improvements to our already established manufacturing methods allow you to acquire longer, purer oligos for your research.

We can help you optimize your panel with NGS functional testing.

Shipping and storage recommendations:

  • Shipped in buffer at reaction-ready concentrations
  • Short-term storage, up to 6 weeks: room temperature (15–25°C) or 4°C
  • Long-term storage, at least 24 months: –20°C

Robust coverage for multiplexed captures

NGS Discovery Pools produce consistent coverage for a range of multiplexed captures (Figure 1).

Figure 1. NGS Discovery Pools show robust capture for single to 20-plex captures. The custom built Pan-Cancer Panel (424 kb) was used to capture different samples using the standard IDT protocol. 92.5% of the target space was covered between 0.5–2X the mean.

Even coverage across targets

NGS Discovery Pools were tested with libraries created from human genomic DNA (Coriell). 500 ng library per sample was used to capture using the standard IDT protocol. The enriched libraries were sequenced on a NextSeq® instrument (Illumina) as indicated and standard Picard Metrics calculated. Data shown is the average for an 8-plex capture (Table 1).

Table 1. NGS Discovery Pools custom panel performance.

 PanelPicard metrics
NameTarget size (kb)Probe countGenes targetedReads per sample (150 bp read length)% selected basesMean target coverage% target at 30+ XDuplicate rate %
Cancer hotspot5252510100,00084.39899.82.0
Pan-cancer4684961125900,00085.810298.11.5
Pan-cancer plus80278201271,300,00085.59896.81.4