Convenient mismatch endonuclease assays that are based on analysis of PCR products spanning your region of interest
For estimating on-target editing efficiency or assessing known off-target effects in your CRISPR experiments, we recommend using the Alt-R Genome Editing Detection Kit (also compatible with ZNF- and TALEN-driven genome editing systems). This T7 endonuclease I mismatch cleavage assay is ideal for directly analyzing your reaction products to detect multiple-base insertions and deletions.