Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Yes, for low-plex pooling (<5 libraries) follow the recommendations provided in Appendix A of the xGen™ Normalase Module Protocol.
If you have a higher volume of the final pool than the volume produced by the standard protocol (5 µL/sample), follow the recommendations provided in Appendix B. These pooling recommendations can help with sequencing on a NovaSeq® and the capability to load a single pool over multiple lanes or flow cells.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.