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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Are there specific recommendations for alternative pooling strategies?
Yes, for low-plex pooling (<5 libraries) follow the recommendations provided in Appendix A of the xGen™ Normalase Module Protocol.
If you have a higher volume of the final pool than the volume produced by the standard protocol (5 µL/sample), follow the recommendations provided in Appendix B. These pooling recommendations can help with sequencing on a NovaSeq® and the capability to load a single pool over multiple lanes or flow cells.