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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Are there specific recommendations for low-complexity samples when using the Lotus DNA Library Prep Kit?

The Lotus DNA Library Prep Kit works well with human genomic DNA and bacterial strains, but it also can be used with low-complexity samples, such as small viral genomes.

However, these low-complexity samples may become over-fragmented if standard fragmentation parameters are used. Therefore, shorter fragmentation times may be required for these sample types to achieve the desired average fragment length. Alternatively, fragmentation can be carried out at a lower temperature to avoid over-fragmentation.