Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can I do a restriction digest directly on my gBlocks™ Gene Fragment prep?

Yes, the yield provided is more than sufficient for a standard digestion/ligation reaction. You will need to add the restriction sites to each end of your gBlocks Gene Fragment plus six additional flanking bases. Many restriction sites need a short DNA stretch upstream of the recognition site to grab onto, to digest efficiently. You can use any 6 bp sequence that has balanced GC content and is not repetitive.



Tags:
iGEM