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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Can I use my own library amplification primers or indexing amplification primers for Normalase™ PCR?
No, Normalase PCR requires replacing your amplification primers to condition your libraries for the downstream enzymology. Reagent R5 replaces standard library amplification primers for those libraries indexed by adapter ligation.
Reagent R6 with xGen™ Normalase CDI primers, or Reagent R7 with Normalase UDI primers, replace your indexing primers for libraries indexed by PCR.