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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can I use RNA with the xGen Prism DNA Library Prep Kit?

The xGen Prism DNA Library Prep Kit can be used for RNA-seq applications. Before sequencing and library construction with the xGen Prism DNA Library Prep Kit, input RNA must be fragmented to an appropriate insert size (150–300 bp), converted to double-stranded cDNA, and purified. Due to inherent inefficiencies in cDNA synthesis and purification, we recommend using inputs of 5–250 ng of RNA.