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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Can IDT make a DNA oligo with a mixture of phosphodiester and phosphorothioate bonds?
Phosphorothioate bonds can be incorporated throughout an oligo by placing an asterisk (*) between the two bases where you would like such modified bonds to appear. As an example, your sequence may look something like this: A*C*T*GCATCAGCCTGCC*A*T*C.
Such oligos must be ordered at 100 nmol scale or higher. Phosphorothioate bonds introduced between the last nucleotides at the 5'- or 3'-end of the oligo inhibit exonuclease degradation.