Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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Can more than 100 µL of rhAmpSeq library pool be used in a single AMPure clean up?

Although 100 µL is recommended, up to 200 µL of rhAmpSeq library pool can be cleaned up in a single reaction. The volume of AMPure® XP beads (Beckman Coulter) should be adjusted so that an equal ratio (1X) of library pool to AMPure XP beads is achieved.

Note: increased magnet incubation times may be necessary for larger volumes.