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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do annealed double-stranded oligos need to be modified prior to ligation into the appropriate restriction site of my cloning vector?

Our chemical synthesis results in a hydroxyl group on both the 3' and 5' terminus of the oligo. DNA ligase requires a 5' phosphate and a 3' hydroxyl group for activity, so a 5' phosphate will need to be added if the intent is to ligate the oligo. This can be requested upon order using the modification code /5Phos/ in the sequence of your oligo. Alternatively, this modification can be added enzymatically through a Polynulcleotide Kinase (PNK) reaction.

Custom DNA & RNA

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.