Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Do custom oligos come with 5’ phosphorylation?
Synthetic DNA and RNA are synthesized with 5' and 3' OH groups. If your application requires a 5' phosphate group, such as a ligation reaction, include a phosphorylation modification as part of your sequence. The modification code for 5' phosphorylation is /5Phos/ and would be inserted into your sequence at the 5' end.