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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Do I have to use the Normalase™ Module to finish my xGen™ Amplicon libraries?
No.
After the Indexing PCR step and the final bead-based size selection/cleanup, xGen Amplicon libraries may be quantified with conventional methods such as Qubit™, Bioanalyzer™ (Aligent), or qPCR and normalized by manual pooling.
Alternatively, they can be normalized enzymatically with the included xGen Normalase reagents.
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.