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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Do I need library quantification after amplification and before Normalase™ I enzyme?
No, it is not necessary to quantify libraries before Normalase™ I enzyme if the libraries are all known to meet the 6 nM or 12 nM yield threshold. For example, if your library preparation and your standard nucleic acid sample inputs consistently yield 20 nM, quantification can be skipped.
Quantification is only required when library yields are unknown, and potentially when they are under the 6 nM or 12 nM minimum threshold.
For quality control purposes, we recommend quantifying libraries after Normalase PCR using a validated qPCR assay or fluorometric quantification assay.
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.