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Do I need to amplify my library when using the xGen Prism DNA Library Prep Kit?
Yes, library amplification is required to generate libraries with the xGen Prism DNA Library Prep Kit. The ligation steps attach partial adapter sequences, and amplification using the xGen Unique Dual Index Primer Pairs is therefore required to incorporate sample indexing sequences and the P5 and P7 sequences, which attach the library to the sequencing instrument flow cells.