Get Help Sign In

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do I need to form my gRNA/RNP complexes separately before multiplexing?


Forming the crRNA:tracrRNA:Cas9 ribonucleoprotein complex prior to mixing multiple crRNAs helps prevent undesireable interactions between different crRNAs before they have an opportunity to correctly hybridize with tracrRNA.