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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do I need to shear or fragment my DNA before using the xGen™ cfDNA & FFPE Library Prep Kit?

DNA has to be an appropriate size before library construction with the xGen™ cfDNA & FFPE Library Prep Kit.  

For genomic DNA or DNA derived from formalin-fixed, paraffin-embedded (FFPE) samples, we recommend using Covaris shearing to attain average insert sizes of 150–300 base pairs. 

As cell-free DNA (cfDNA) typically has an average size of 160 base pairs, no further fragmentation is required. 

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.