Get Help Sign In
ProcessingProcessing

Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do I need to shear or fragment my DNA before using the xGen Prism DNA Library Prep Kit?

DNA has to be an appropriate size before library construction with the xGen Prism DNA Library Prep Kit. For genomic DNA or DNA derived from formalin-fixed, paraffin-embedded (FFPE) samples, we recommend using Covaris shearing to achieve average insert sizes of 150–300 base pairs. As cell-free DNA (cfDNA) typically has an average size of 160 base pairs, no further fragmentation is required.



Tags:
xgen

Search all FAQs: