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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do the ends of synthetic RNA oligos have the correct chemistry for ligation?

Oligonucleotides from IDT have terminal hydroxyl groups on both ends. If the oligos will be used in ligation reactions, you will need to request a 5' terminal phosphate as a modification, or you can add it enzymatically using a kinase reaction.

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