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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Do the positive and negative controls in the TriFECTa® Kit include primers and RT-PCR materials?

The kit does not include primers or PCR reagents for quantification of gene silencing. Separately, we offer primer sets for targeting human or mouse HPRT for use in SYBR® Green–based qPCR assays to monitor RNAi function following transfection with the HPRT-S1 Positive Control DsiRNA.

For other human, mouse, or rat targets, consider using a Predesigned PrimeTime® qPCR Assay. For additional species, you can easily design a custom PrimeTime qPCR Assay using our free, online PrimerQuest® Tool.

The TriFECTa Kit includes:

  • 3 target-specific Dicer-Substrate siRNAs (DsiRNAs, 2 nmol each)
  • 1 negative control DsiRNA (NC1, which does not target any human, mouse, or rat sequence)
  • 1 positive control DsiRNA (HPRT-S1 DS Positive Control)
  • 1 fluorescent transfection control DsiRNA (TYE 563-labeled transfection control)
  • Nuclease-Free Duplex Buffer

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.