Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Does my probe sequence need to be modified to convert it to a ZEN double-quenched probe?
The sequence does not need to be altered to accommodate an internal ZEN modification. IDT has tested many assays with direct substitution of ZEN/IBFQ into the probe sequence and has found no performance issues. Note that when ordering a ZEN modification, both the assay and probe ordering tools will automatically insert the ZEN quencher in the appropriate location. Read more about the uses of the ZEN secondary quencher at Modification Highlight: ZEN™ Internal Quencher.