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Bases added to the 3’ termini of DNA fragments during the Adaptase reaction contain unmethylated cytosines, which adds both artifactual sequence and methylation information to the dataset. Therefore, tail trimming of xGen™ Methylation-Sequencing DNA libraries is required to attain sufficient mapping efficiency and precise methylation information.
See our technical note, Tail Trimming for Better Data for further details on the tail and how to trim it.
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