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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Does the Adaptase™ tail need to be bioinformatically trimmed for methylation sequencing?
Yes.
Bases added to the 3’ termini of DNA fragments during the Adaptase reaction contain unmethylated cytosines, which adds both artifactual sequence and methylation information to the dataset. Therefore, tail trimming of xGen™ Methylation-Sequencing DNA libraries is required to attain sufficient mapping efficiency and precise methylation information.
See our technical note, Tail Trimming for Better Data for further details on the tail and how to trim it.
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.