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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Does the xGen™ Amplicon 16S-SNAPP pipeline assemble reads to full-length 16S sequences?

The 16S-SNAPP pipeline attempts to generate consensus sequences from reads mapped to the same reference sequences.

Depending on differential primer efficiency, the completeness of the reference set, the sequencing depth and read length, the 16S-SNAPP pipeline is able to generate consensus sequences of various lengths and approaching near full-length sequences of specific variable regions.

However, the panel lacks phasing and amplicon sequences cannot be linked together due to the complex composition of a metagenomic sample.

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