Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Does the xGen™ Broad-Range RNA Library Prep Kit work with damaged or degraded RNA?
Yes.
Refer to the xGen Broad-Range RNA Library Prep Kit Protocol (Appendix B) for details regarding the changes to RNA fragmentation time and appropriate SPRI bead ratios for samples with RIN scores lower than 7.
Note: We do not recommend generating libraries from starting RNA with an RIN score < 2.
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.