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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Does the xGen™ RNA Library Prep Kit include upstream poly(A) enrichment or ribodepletion modules?
No, this kit takes as input one of the following:
1. Total RNA
2. mRNA that has already been enriched by poly(A) selection
3. RNA that has already been depleted of rRNA
The xGen RNA Library Prep Kit begins with an RNA fragmentation and reverse transcription step. Therefore, if mRNA enrichment or ribodepletion is desired, one of the kits below is required and recommended for pre-processing. These kits have been tested for compatibility with the xGen RNA Library Prep Kit.
Note: View this xGen RNA Library Prep Kit Protocol for instructions on matching the output of these upstream modules with the initial steps of the xGen RNA Library Prep Kit.
1. Total RNA
2. mRNA that has already been enriched by poly(A) selection
3. RNA that has already been depleted of rRNA
The xGen RNA Library Prep Kit begins with an RNA fragmentation and reverse transcription step. Therefore, if mRNA enrichment or ribodepletion is desired, one of the kits below is required and recommended for pre-processing. These kits have been tested for compatibility with the xGen RNA Library Prep Kit.
Note: View this xGen RNA Library Prep Kit Protocol for instructions on matching the output of these upstream modules with the initial steps of the xGen RNA Library Prep Kit.
| Method | Recommended kit | Alternate kit |
|---|---|---|
| Poly(A) mRNA enrichment | NEBNext® Poly (A) mRNA Magnetic Isolation Module Cat. No. E7490 |
Lexogen Poly (A) RNA Selection Kit Cat. No. 039.100 |
| Ribodepletion | Lexogen RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat) Cat. No. 037.24/96 |
NEBNext rRNA Depletion Kit (Human/Mouse/Rat) Cat. No. E6310S/L/X |
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Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.