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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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Does the xGen™ Broad-Range RNA Library Prep Kit include upstream poly(A) enrichment or ribodepletion modules?
No, this kit takes as input one of the following:
- Total RNA
- mRNA that has already been enriched by poly(A) selection
- RNA that has already been depleted of rRNA
The xGen Broad-Range RNA Library Prep Kit begins with an RNA fragmentation and reverse transcription step. Therefore, if mRNA enrichment or ribodepletion is required, see the xGen Broad-Range RNA Library Prep Kit Protocol (Appendix A) for kits that have been tested for compatibility with this protocol.
Also, refer to the protocol for details on matching the output of these upstream modules with the initial steps in the xGen Broad-Range RNA Library Kit instructions.
| Method | Recommended kit | Alternate kit |
|---|---|---|
| Poly(A) mRNA Enrichment | NEBNext® Poly (A) mRNA Magnetic Isolation Module Cat. No. E7490 |
Lexogen Poly (A) RNA Selection Kit Cat. No. 039.100 |
| Ribodepletion | Lexogen RiboCop rRNA Depletion Kit V1.2 (Human/Mouse/Rat) Cat. No. 037.24/96 |
NEBNext rRNA Depletion Kit (Human/Mouse/Rat) Cat. No. E6310S/L/X |