Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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For genotyping applications, what is the recommended minimum sequencing coverage per assay?

We recommend a minimum of 20 reads to accurately call genotypes in diploid species with heterozygosity (i.e., 50% allele frequencies). For polyploid species or somatic mutations with lower allele frequencies, your minimum coverage may need to be higher.