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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
For variant calling applications, what is the recommended minimum sequencing coverage per assay?
The minimum coverage per assay will depend on the target variant allele frequency (VAF) of the experiment. Lower VAF values will require more reads to see rare variants.
Assuming a 50% VAF, we recommend calling SNPs at targets covered by a minimum of 20 reads.