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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How are IDT HDR donor oligos modified?

We provide 3 options:

  1. Alt-R HDR modification. This is our recommended option for the best HDR performance. It includes a proprietary modification pattern developed through extensive testing to increase donor oligo stability and enable the highest rate of repair.
  2. Phosphorothioate (PS) bond modification. There are 4 PS bonds total; the first 3 and last 3 bases of the HDR donor oligo incorporate these bonds in the sequence. Example: N*N*NNNN…NNNN*N*N (* = phosphorothioate bond; N = DNA base)
  3. No modification. Standard DNA.


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.