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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How can I determine if a hairpin in my oligo is too strong to allow hybridization?
Using free OligoAnalyzer™ software, part of the IDT SciTools™ programs, enter your oligonucleotide sequence and choose “Hairpin.” The software will generate a series of possible hairpin structures.
You can arrange these structures in order of decreasing melting temperature (Tm). If the highest hairpin Tm is at or above your annealing temperature, that hairpin is likely to impede hybridization.
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