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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How can I tell if my oligos successfully annealed?
The hybridized oligos or DNA duplex can be run on a non-denaturing gel with appropriate molecular weight markers.
Alternatively, run the duplexed oligo side by side with a single-stranded oligo. If annealing was successful, the double-stranded band will be shifted up from the single-stranded band.
Use UV back shadowing or a stain such as SYBR™ Gold to visualize the bands. Ethidium bromide may not sufficiently stain the single-stranded DNA to make it visible.