Processing
Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How can I tell if my oligos successfully annealed?
The hybridized oligos or DNA duplex can be run on a non-denaturing gel with appropriate molecular weight markers.
Alternatively, run the duplexed oligo side by side with a single-stranded oligo. If annealing was successful, the double-stranded band will be shifted up from the single-stranded band.
Use UV back shadowing or a stain such as SYBR™ Gold to visualize the bands. Ethidium bromide may not sufficiently stain the single-stranded DNA to make it visible.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.