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The hybridized oligos or DNA duplex can be run on a non-denaturing gel with appropriate molecular weight markers.
Alternatively, run the duplexed oligo side by side with a single-stranded oligo. If annealing was successful, the double-stranded band will be shifted up from the single-stranded band.
Use UV back shadowing or a stain such as SYBR™ Gold to visualize the bands. Ethidium bromide may not sufficiently stain the single-stranded DNA to make it visible.
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