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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I calculate the Tm of my oligo if I want to incorporate mismatches?

The free, IDT online OligoAnalzyer tool, accessed from the SciTools tab on the IDT website (, allows researchers to set mismatches. Enter your sequence into the input box, and then click the Tm Mismatch button. Click on the base you wish to change and then set the mismatch base using the drop down menu. After clicking <calculate>, the exact match and mismatch Tms will be displayed.

Custom DNA & RNA

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