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How do I identify CRISPR editing off-target sites?
The methods used to nominate off-target sites fall into two categories:
Empirical methods (e.g., GUIDE-seq , CIRCLE-seq , Digenome-seq , DISCOVER-seq , or other empirical methods) use wet lab experiments to identify off-target sites.
In silico tools (e.g., IDT CRISPR-Cas9 guide RNA design checker) attempt to identify off-target, double-stranded breaks that may occur in the genome.
Of note, there are significant differences between the off-target sites that are nominated using biochemical (in vitro), cell-based, or in vivo methods.