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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I input the include/exclude regions on the PrimerQuest "Advanced" menu?

Only one included region may be defined per design in the PrimerQuest design tool. The included region is entered as a range; for example, 1200-1500. Multiple excluded regions are allowed per design in PrimerQuest. The "Excluded Region List" is a comma separated list of ranges. For example, 1200-1500, 1600-1700, 1850-1950. Find both of these parameter fields on the main page of the PrimerQuest design tool under the "Custom Target Region" section. Read more about how to use the PrimerQuest tool in PrimerQuest® Design Tool: From Basic to Highly Customizable Designs.