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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I know if I over-amplified my sequencing library?

Library over-amplification results in the formation of heteroduplex and daisy-chained products in which strands of library molecules with non-complementary inserts are annealed to each other via the adapter sequences. When analyzed by electrophoresis, these heteroduplex and daisy-chained products migrate slower and appear as an additional peak larger than the expected library size.