Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How do I resuspend my oligos?

We find it convenient to initially make a at 100 µM freezer stock, which should be thawed relatively infrequently to make more dilute aliquots for short term use. Dissolve the oligo in TE (10 mM Tris pH 8.0, 0.1 mM EDTA). Use the IDT Resuspension Calulator under the "SciTools®" tab of the IDT website for help determining volume. Alternatively, sterile dH2O can be used. DNA kept frozen in a nuclease-free environment should be stable for years.

Categories:
Custom DNA & RNA