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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I resuspend my oPools™ Oligo Pools to my desired concentration?

Calculate the appropriate resuspension volume for your oPools product with the  IDT Resuspension Calculator.

First, convert the oPool scale—provided in pmol—to a nmol quantity.

If your desired final concentration is µM per oligo, use this equation to determine your quantity:

pmol per oligo / 1000 = # nmol per oligo

Example 1. Final concentration of 1 µM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol 
Final concentration: 1 µM
Resuspension calculator (Figure 1):

  • Enter the quantity that you calculated
  • Enter your final concentration
  • Select Sgl-Stranded DNA (single-stranded DNA)
  • Click Calculate

Screen capture: resuspension results for 1 µM per oligo final concentration

Figure 1. 50 pmol oPools Oligo Pool resuspended to 1 µM per oligo.

If your desired final concentration is nM per oligo, you can convert using this equation: 

nM concentration / 1000 = # µM

Example 2. Final concentration of 100 nM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol 
Final concentration in µM: 100 nM  / 1000 = 0.1 µM
Resuspension calculator (Figure 2):

  • Enter the quantity that you calculated 
  • Enter your final concentration per oligo that you calculated
  • Select Sgl-Stranded DNA (single-stranded DNA) 
  • Click Calculate

Screen capture: resuspension results for 100 nM per oligo final concentration

Figure 2. 50 pmol oPools Oligo Pool resuspended to 100 nM per oligo.

If your desired final concentration is per pool, use this equation: 

(pmol per oligo x # of oligos in pool) / 1000 = # nmol per pool

Example 3. Final concentration of 1 µM per pool.
Scenario: 50 pmol oPools Oligo Pool containing 100 oligos
Quantity: (50 pmol x 100 oligos) / 1000 = 5 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 3):

  • Enter the quantity that you calculated
  • Enter your final concentration
  • Select Sgl-Stranded DNA (single-stranded DNA)
  • Click Calculate

Screen capture: resuspension results for 1 µM per pool final concentration

Figure 3. 50 pmol oPools Oligo Pool containing 100 oligos resuspended to 1 µM per pool.


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Custom DNA & RNA

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