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Calculate the appropriate resuspension volume for your oPools product with the IDT Resuspension Calculator.
First, convert the oPool scale—provided in pmol—to a nmol quantity.
If your desired final concentration is µM per oligo, use this equation to determine your quantity:
pmol per oligo / 1000 = # nmol per oligo
Example 1. Final concentration of 1 µM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 1):
Figure 1. 50 pmol oPools Oligo Pool resuspended to 1 µM per oligo.
If your desired final concentration is nM per oligo, you can convert using this equation:
nM concentration / 1000 = # µM
Example 2. Final concentration of 100 nM per oligo.
Scenario: 50 pmol oPools Oligo Pool
Quantity: 50 pmol oPools Oligo Pool / 1000 = 0.05 nmol
Final concentration in µM: 100 nM / 1000 = 0.1 µM
Resuspension calculator (Figure 2):
Figure 2. 50 pmol oPools Oligo Pool resuspended to 100 nM per oligo.
If your desired final concentration is per pool, use this equation:
(pmol per oligo x # of oligos in pool) / 1000 = # nmol per pool
Example 3. Final concentration of 1 µM per pool.
Scenario: 50 pmol oPools Oligo Pool containing 100 oligos
Quantity: (50 pmol x 100 oligos) / 1000 = 5 nmol
Final concentration: 1 µM
Resuspension calculator (Figure 3):
Figure 3. 50 pmol oPools Oligo Pool containing 100 oligos resuspended to 1 µM per pool.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.