Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

FAQ categories

CRISPR genome editing 
Custom DNA & RNA 
Functional genomics 
Genes & gene fragments 
Next generation sequencing 
Optional services 
qPCR & PCR 
Reagents & kits 

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How does the efficiency of RNA synthesis compare with DNA?

RNA synthesis is slightly over 98% efficient, while DNA couples at roughly 99.2%. As with DNA, short RNA oligos will include a low percentage of truncated products as a result of the less than 100% efficiency of coupling. These oligos may be sufficiently pure for your application without requiring additional purification. For sensitive RNA applications, such as silencing, we strongly recommend purification.

Categories:
Custom DNA & RNA

Tags:
dna synthesis oligonucleotide rna synthesis