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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
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How many cycles of library amplification should be used for the xGen™ DNA Library Prep Kits?
Below are the recommended minimum PCR cycles for direct sequencing of libraries prepared from high-quality genomic DNA for each xGen DNA Library Prep Kits.
When using indexing primer kits, a minimum number of 3 PCR cycles is required to complete adapter sequences, irrespective of whether enough library is available after ligation.
For samples of compromised quality, additional cycles may be required.
xGen DNA Library Prep Kit EZ
| Input material (ng) | Minimum PCR cycles |
|---|---|
| ≥100 | 3 |
| 25 | 5 |
| 10 | 7 |
| 1 | 10 |
xGen DNA Library Prep Kit MC
| Number of cycles required to generate | ||
|---|---|---|
| Input material (ng) |
100 ng library | 1 µg library |
| 1000 | 0* | 2 |
| 500 | 0* | 2–4 |
| 250 | 0–2* | 4–6 |
| 100 | 2–3 | 6–7 |
| 50 | 3–5 | 7–8 |
| 25 | 5–7 | 8–10 |
| 10 | 7–9 | 11–13 |
| 5 | 9–11 | 13–14 |
| 2.5 | 11–13 | 14–16 |
| 1 | 13–15 | 17–19 |
Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.