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Below are the recommended minimum PCR cycles for direct sequencing of libraries prepared from high-quality genomic DNA for each xGen DNA Library Prep Kits.
When using indexing primer kits, a minimum number of 3 PCR cycles is required to complete adapter sequences, irrespective of whether enough library is available after ligation.
For samples of compromised quality, additional cycles may be required.
xGen DNA Library Prep Kit EZ
Input material (ng) | Minimum PCR cycles |
---|---|
≥100 | 3 |
25 | 5 |
10 | 7 |
1 | 10 |
xGen DNA Library Prep Kit MC
Number of cycles required to generate | ||
---|---|---|
Input material (ng) |
100 ng library | 1 µg library |
1000 | 0* | 2 |
500 | 0* | 2–4 |
250 | 0–2* | 4–6 |
100 | 2–3 | 6–7 |
50 | 3–5 | 7–8 |
25 | 5–7 | 8–10 |
10 | 7–9 | 11–13 |
5 | 9–11 | 13–14 |
2.5 | 11–13 | 14–16 |
1 | 13–15 | 17–19 |
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.