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It depends upon your method for delivery, reaction volume, and Cas9 concentration.
As an example:
|Tube size||Delivery method||Reaction volume||Cas9 concentration||Number of reactions|
|100 µg||Lipofection||150 µL||10 nM||~400|
|100 µg||Electroporation||10 µL||3 µM||~20|
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