Frequently asked questions

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How many reactions will I be able to do with 25 nmol PCR primers?

Most PCR reactions use 0.1−0.5 μM primer. Assuming a maximum concentration of 0.5 μM and a reaction volume of 20 μL, each reaction will require 10 pmol oligonucleotide primer. For a typical 25mer oligonucleotide, 1 OD is equivalent to approximately 4 nmol, or 4000 pmol. Guaranteed yield for most constructs on the 25 nmol scale is 3 ODs. Therefore, with even the minimum yield from a 25 nmol synthesis, you should be able to perform 1200 PCR reactions. In summary: 3 ODs of a 25mer ≈ 12 nmol = 12,000 pmol = 1200 reactions.

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Custom DNA & RNA