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The rhAmpSeq Library Preparation Protocol takes 4–6 hours with 2.5–4.5 hours of hands-on time.
The rhAmpSeq High Throughput Library Preparation Protocol takes approximately 4–4.5 hours with 1–1.5 hours of hands-on time.
Note: Times listed are for processing 12–96 samples using manual pipetting, including reaction setup, cleanup, library quantification, and normalization steps. The time for either protocol will vary depending on the number of samples being processed.