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Frequently asked questions
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How often are the PAM sequences presented in the mammalian genome on average?
Using the frequency of ‘GG’ = 5.21% in the reference human genome  there would be an expected 161,284,793 NGG PAM sites in the human genome, or roughly one ‘GG’ dinucleotide every 42 bases.
Scherer S Cold Spring Harbor Laboratory. Press., A short guide to the human genome2008, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. xiv, 173 p.