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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How sensitive is the rhAmpSeq™ CRISPR Analysis System?
With CRISPAltRations™, the software behind the rhAmpSeq CRISPR Analysis Tool, we analyzed the editing in three experimental replicates of 20 paired, treated, and control Cas9-edited loci. The treated samples had known indel editing levels (0.05, 0.10, 0.20, 0.50, 1.0, 2.0, and 3.4%), while the control samples had noise signals ranging from 0–0.4%.
Using the Fisher’s Exact Test (with a threshold of p<0.5), we show that 95% sensitivity and specificity were obtained for detecting indels down to 0.5%. This analysis assumed that there were:
- more than 5000 paired-end reads in both the treatment and control samples
- ·normal levels of background indel noise (<0.4% indels)
- treatment sample loci with editing >0.5%
- libraries prepared with the rhAmpSeq CRISPR Library Kit and sequencing data analyzed by the rhAmpSeq CRISPR Analysis Tool (powered by CRISPAltRations®).
Categories:
CRISPR genome editing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.