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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How should I resuspend the Alt-R HDR Donor Block?

Follow these steps to resuspend Alt-R™ HDR Donor Blocks:

  1. Before opening the tube, spin in a microcentrifuge for 3–5 sec to ensure the Alt-R HDR Donor Blocks DNA is in the bottom of the tube.
  2. Add molecular grade water, or a buffer such as IDTE, to a final stock concentration of
    500 ng/µL, or a concentration appropriate for your planned experiment.
  3. Briefly vortex.
  4. Incubate at approximately 50°C for 20 min. Heating the tube will ensure the water or buffer contacts the tiny pellet, even if it is stuck to the side of the tube. Thus, this step increases the likelihood that the entire pellet will be resuspended.
  5. Vortex and centrifuge for 3–5 sec. Due to low resuspension volumes required for this product, thorough vortexing or pipetting is recommended to ensure the sides of the tube have been thoroughly exposed to your buffer of choice.
  6. Verify the final concentration using the NanoDrop™ (Thermo Fisher Scientific), Qubit® instruments (Thermo Fisher Scientific), or another spectrophotometer. Depending on your analysis method, you may want to make a dilution of your sample for the concentration check to conserve material.

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.