Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

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How will my Custom NGS Adapters be delivered?

Custom NGS Adapters are provided as either single or duplexed oligos in tubes or plates, depending on the type of adapter selected.

Adapter Format Solution
  • Nextera™–Compatible Indexing Primers
  • TruSeq™–Compatible Primers (use with TruSeq™–Compatible Stubby Adapters)
Oligo mixes IDTE (1X TE), pH 8.0 (IDT)
  • TruSeq™–Compatible Full-length Adapters
  • TruSeq™–Compatible Stubby Adapters (use with TruSeq™–Compatible Primers)
Duplexed oligos Nuclease-free Duplex Buffer (30 mM HEPES, pH 7.5; 100 mM potassium acetate; IDT)

The amount provided is based on the selection of mass (2, 8, or 24 nmol) or reaction number (16 or 96 reactions; where 1 reaction = 0.025 nmol for Nextera™–Compatible Indexing Primers; 1 reaction = 0.50 nmol for TruSeq™–Compatible Primers; and 1 reaction = 0.075 nmol for TruSeq™–Compatible Full-length or Stubby Adapters).

(Visit the IDT Usage, warranty & licensing page for specific information about Illumina trademarks.)


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