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Frequently asked questions
Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
How will my Custom NGS Adapters be delivered?
Custom NGS Adapters are provided as either single or duplexed oligos in tubes or plates, depending on the type of adapter selected.
| Adapter | Format | Solution |
|---|---|---|
|
Oligo mixes | IDTE [1X TE (10 mM Tris, 0.1 mM EDTA)], pH 8.0 (IDT) |
|
Duplexed oligos | Nuclease-free Duplex Buffer (30 mM HEPES, pH 7.5; 100 mM potassium acetate; IDT) |
The amount provided is based on the selection of mass (2, 8, or 24 nmol), or reaction number (16 or 96 reactions; where 1 reaction = 0.050 nmol for Nextera™–Compatible Indexing Primers; 1 reaction = 0.050 nmol for TruSeq™–Compatible Primers; and 1 reaction = 0.075 nmol for TruSeq™–Compatible Full-length or Stubby Adapters).
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Categories:
Next generation sequencing*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.